Purpose of the study: To evaluate an expression level of brain-derived neurotrophic factor (BDNF) in highly sensitive to hypoxia neuronal populations of male and female rats after total ischemia.
Materials and Methods: Cardiac arrest in adult albino male and female rats (n=25) was evoked by intrathoracic clamping of cardiac vascular bundle for 10 min. Sham-operated animals served as control (n=10). Animals were resuscitated with the aid of chest compressions combined with mechanical air ventilation by “Animal Respirator” (SMT Geratehandel) followed by intratracheal administration of 0.1 mg/kg adrenaline solution.
The pyramidal neurons of the hippocampus (field CA4) and Purkinje cells of the cerebellum were analysed on the 1, 4, 7 and 14th day after resuscitation.
The expression of BDNF protein was determined by peroxidase-antiperoxidase method using polyclonal antibodies to BDNF (Santa Cruz, USA) and LSAB TM +Kit visualizing system (DAKO, Denmark).
The intensity of BDNF expression in the neuron's cytoplasm was assessed from the mean optical density using image analysis software ImageJ 1.48v. A number of neurons with different expression level of BDNF as well as a total number of neurons (per 1 mm of their layer length) were evaluated.
Statistical data processing was performed using Statistica 7.0. The significance of differences was assessed by Student's t-test, Mann-Whitney test, Kolmogorov-Smirnov test.
Conclusion: Cardiac arrest causes more pronounced neuronal injury in male rats compared to female ones as well as shifts in the expression level of BDNF. There are interrelation between BDNF expression shifts and the postresuscitative neuronsal loss. The high level of BDNF protein enhances sustainability of neurons to ischemia-reperfusion.