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Oct 24, 2018

7th Congress of the European Academy of Paediatric Societies

10 - IDENTIFICATION OF A PANEL OF SERUM PROTEIN MARKERS IN EARLY STAGE OF PEDIATRIC SEPSIS AND ITS VALIDATION IN A COHORT OF PATIENTS

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biomarker

sepsis

pediatric

Abstract

Abstract

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Keywords

biomarker

sepsis

pediatric

Abstract

Background and Aims Sepsis is a life-threatening disorder being a major cause of mortality worldwide. Biomarkers to diagnose sepsis may allow early intervention and reduce the risk of death. The aim of this study was to improve the diagnosis of severe sepsis in children by identifying and validating new biomarkers Methods: Prospective study of a single center. Serum samples were obtained from 40 septic pediatric patients and 24 healthy children. From this cohort, 10 septic patients and 5 healthy donors were selected for the proteomic study Protein analysis was performed by mass spectrometry and validation of the identified proteins was carried out by Enzyme-Linked ImmunoSorbent Assays Results: Mass-spectrometry analysis revealed 44 significant deregulated proteins between patients and healthy donors. After applying some selection criteria, 4 proteins were selected for the validation process: Lactoferrin (LTF), Serum Amyloid A1 (SAA-1), Leucine-Rich alpha-2-Glycoprotein (LRG1) and Complement Factor B (CFB).  Moreover, sCD25 and sCD163 were analyzed in the same cohort of patients and healthy donors. All these proteins were upregulated in septic patients´ serum when they were compared with healthy donors´ serum with the exception of CFB.  Further analysis revealed that SAA-1, LRG1 and sCD25 have high sensitivity and specificity with a high area under the receiver operating characteristic that make them promising biomarkers in the diagnosis of pediatric sepsis Conclusions: We have identified a panel of three potential sepsis biomarkers biologically connected and validated in a group of pediatric patients with sepsis, whose analysis could be considered as a complementary tool for the diagnosis of sepsis

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© Copyright 2019 Morressier GmbH.
All rights reserved.