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Nov 9, 2018

COGI-2018

17 - TRANSCRIPTIONAL ANALYSIS OF GENES ENDOCING PROTEINS PRESENTED IN THE EXTRACELLULAR SPACE OF ENDOMETRIAL STROMAL CELLS IN HUMAN

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human endometrial stromal cells

extracellular matrix

cell-to-cell interaction

integrin

Abstract

Abstract

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Keywords

human endometrial stromal cells

extracellular matrix

cell-to-cell interaction

integrin

Abstract

Problem statement: In in-vivo endometrium, endometrial cells experience dramatic alterations during the menstrual cycle by a variety of hormones secreted from sexual organs. These hormonal regulation make it difficult to study physiological and pharmacological aspects of endometrial biology. Therefore, organization of artificial uterine tissues through construction of non-cellular niche mimicking microenvironment enclosing endometrium is important for eliminating chaotic treatment effects detected in the implantation-related studies. Accordingly, as a step towards constructing a precisely defined three dimensional microenvironment engineering extracellular signaling to regulate physiological characteristics of endometrial cells derived from human uterus, information on microenvironment factors presented in the extracellular space of human endometrial stromal cells (hESCs) should be requested. Methods: we conducted transcriptional analysis of genes encoding extracellular matrix, cell-to-cell interaction, and integrin proteins expressed in hESCs. Each transcriptional level was measured by real-time polymerase chain reaction (RT-PCR). Results: In case of extracellular matrix proteins, 2 (COLI and COLIII) genes showed significantly higher transcriptional level than the other genes, whereas 7 (FN, NID, LN, TN, ELN, COLIV and COLⅤ) genes were weakly transcribed and no transcription of COLII and COLXI was detected. Moreover, in the cell-to-cell interaction proteins, CDH2 showed significantly the strongest transcription compared to the other genes and 7 (CDH1, CDH3, DSG2, ICAM1, HSPG2, VCAN and SDC1) genes showed extremely low transcriptional level with no transcription of DSC3, PECAM1 and SELL. Finally, among total 25 integrin subunit genes, 4 integrin alpha subunit (ITGA1, ITGA3, ITGA4 and ITGAV) and 3 integrin beta subunit (ITGB1, ITGB3 and ITGB5) genes showed significantly increased transcriptional level compared to the other genes, whereas 10 integrin alpha subunit (ITGA2, ITGA5, ITGA6, ITGA7, ITGA8, ITGA9, ITGA10, ITGA11, ITGAL and ITGAX) and 3 integrin beta subunit (ITGB2, ITGB4 and ITGB8) genes were weakly transcribed with no transcription of ITGAM, ITGAD, ITGAE, ITGB6 and ITGB7. Conclusion: we could identify types of extracellular matrix (COLI and COLIII), cell-to-cell interaction (CDH2), and integrin protein (ITGA1, ITGA3, ITGA4, ITGAV, ITGB1, ITGB3 and ITGB5) -encoding genes significantly up-regulated transcriptionally in hESCs. We have no conflict of interest to declare. Funding: NRF-2017R1A2B4009777

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© Copyright 2019 Morressier GmbH.
All rights reserved.