In central-west region of Brazil, where  Paracoccidioides lutzii predominates, has been described low reactivity in immunological tests using Paracoccidioides standard antigen (AgB-339). Here, we aimed to evaluate the performance of exo-antigens from P. lutzii against patient sera from Brazilian central-west region (CWR), and to speculate their influence under PCM immunological diagnose, as well sorological follow-up of these patients. The cell-free culture antigens, known as exo-antigens, were obtained from P. lutzii (ATCC MYA-826), defined as PlAg, and proved by double agar-gel immunodiffusion (DID) test against 53 PCM suspect serum samples from CWR. The PCM confirmation was given in 81.13% of cases (43/53) by direct exam, biopsy specimens or culture.

Exo-antigens from Paracoccidioides lutzii applied to immunodiagnosis of Paracoccidioidomycosis: an report of our experience in Brazil's central-west