The need to remove nitrogen from wastewater for most wastewater treatment plants around the world. Conventional removal of inorganic nitrogen is completed by nitrification-denitrification processes. However, these processes carbon and energy intensive. The other alternative technology is anaerobic ammonium oxidation (Anammox). Since its discovery in early 1990s, Anammox the ideal treatment technology in removal of TIN in wastewater. Anammox is efficient both in removal of TIN and energy consumption as well as no requirement for additional carbon as Anammox takes place in anoxic environment and Anammox bacteria are autotrophs capable of using CO2 as their carbon source. Anammox process shows a promising future in full deployment to remove TIN couples with conventional activated sludge process to remove organic carbon in wastewater treatment plants. However, Anammox technology is in development stage requires more research to fully understanding the process. Anammox bacteria are known for their slow growth rates and widespread inhibited by different factors including various chemicals that are commonly found in wastewater. However, the mechanisms behind this chemical inhibition are not well understood, especially at the molecular level. There are discrepancies in reported inhibiting concentrations of the chemicals, like inhibition by NO2- ranges from 5 to 280 mg N/L depending on different research groups. Our study focuses on mechanisms of the inhibition of Anammox by NO2-, S2- and recalcitrant carbons at the molecular level by investigate of gene expression and metagenomic of the Anammox during the stress tests under an NSF funded project. Results show that ammonia monooxygenase gene expressions increased where the ammonia oxidation (contrary to amoA gene expression) was inhibited under external perturnations listed above. On the other hand, the expression of hyzrazine synthase enzyme responsible for the final step in Anammox decreased substanatially. Currently, serum bottle tests are being conducted to evaluate the kinetics and response of other flanking community members. Preliminary result has shown that with our setup, Anammox activity was not effected by NO2- up to 30 mg N/L but partially inhibited beyond this concentration upto 100 mg/L.

Understanding inhibition of anammox activity by nitrite, sulfide and recalcitrant carbonsat a functional gene level

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.