N-(phosphonomethyl) glycine or glyphosate (GP) is currently one of the most used herbicides worldwide. It is a broad-spectrum, post-emergent, non-selective, systemic herbicide, which effectively kills or suppresses all plant types, including grasses, perennials, vines, shrubs, and trees. IARC classified GP as probably carcinogenic to humans" (Group 2A). GP has been related to kidney diseases worldwide as a chelating agent, also found in different matrices such as soil, air, water, food, human urine, and breast milk.<br/>Some studies have shown the possibility of GP adducts formation with DNA and proteins. There is a debate about whether GP, or its metabolite aminomethyl phosphonic acid (AMPA), is responsible for its carcinogenic effects.<br/>Currently, there are no kinetic or thermodynamic studies showing a possible relation between that info and the alkylating potential of GP, which is a great tool in the epidemiological studies of a specific compound. An effective technique to determine the alkylating potential of GP is the 4-(p-nitrobenzyl) pyridine (NBP) essay. NBP is considered a trap for alkylating agents due to its nucleophilic characteristics similar to those of DNA bases and is known for its ease of reaction with strong and weak alkylating agents.<br/>The present study will be focused to show the use of the NBP essay in order to measure the alkylating activity of GP and a possible relation with its carcinogenic and mutagenic effects. A research route will be presented, together with some preliminary kinetic information, such as rate constants, temperature dependence, and thermodynamic analysis.

Preliminary kinetic study of NBP alkylation by the herbicide glyphosate

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.