The use of higher productivity protein A capture matrices for the purification of monoclonal antibodies (mAbs) is becoming essential due to higher productivity bioreactor technologies. Six different Protein A resins and 3 novel protein A matrices were studied to characterize Dynamic Binding Capacity (DBC), sodium hydroxide (NaOH), resin, and matrix lifetime. Additionally, batch loading, intensified batch, and intensified batch with dynamic flow loading productivities were modeled to compare costs and productivities of these intensified processes to the theoretical costs and productivities of the novel matrices. These data demonstrate novel protein A matrix productivity gains, although substantial in comparison to the current state of batch chromatography, are not as large when compared to existing protein A resins using intensified processes.

Comparison of protein A resins and novel protein A matrices

ACS Fall 2020 Virtual Meeting

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