and 1 other(s)
Objectives: Abrasive particles in toothpastes can interact with oral cells, they can even enter periodontal pockets or extraction sockets in the oral cavity. Aim of this in vitro study was to investigate whether abrasives in toothpastes can cause cytotoxicity and provoke an inflammatory cell response. Methods: Abrasives of commercially available toothpastes with defined RDA values were prepared. L929 murine fibroblasts and RAW264.7 murine macrophages were exposed to various concentrations of abrasives. Cell viability testing, evaluation of gene expression, and immunoassay were performed. Results: We report here that all abrasives, reduced cell viability at 0.2%. Abrasives caused a potent inflammatory response indicated by the increased expression of IL1a, IL1b and IL6 in L929 and RAW264.7 cells. Conclusion: Together these findings suggest that under in vitro conditions abrasives from toothpastes reduce cell viability and provoke an inflammatory response.
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