and 3 other(s)
Aim: The aim of this study was to evaluate the effect of dissociated soft tissue that were relevant to bone regeneration. Methods and Materials: Dissociated soft tissue suspension was prepared from rat palatal mucosa with a tissue dissociative device. In vitro, dissociated suspension or αMEM was added to BMCs. After 2,5 and 7 days in culture, cell proliferation and osteoblastic differentiation were assessed. In vivo, dissociated suspension or sucrose solution combined with hydrogel was put on the bone defect created in rat calvaria. Rats were sacrificed at 1 and 2weeks for radiological and histological analysis. Results: In vitro, BMCs cultured with dissociated suspension exhibited significantly higher cell viability and osteogenic differentiation at day 2. In vivo, the volume of newly formed bone was significantly greater in dissociated suspension group and the defect was filled with new bone at 2 weeks. Conclusion: These results indicated that dissociated mucosa suspension had effect on proliferation and osteoblastic differentiation in early time phase. Moreover, autogenous mucosa promote bone regeneration. These findings might suggested that dissociated suspension induced the synergistic effects of signal molecule from fibroblast and viable fibroblast.
No datasets are available for this submission.
No license information is available for this submission.