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Sep 24, 2018

7th Congress of the European Academy of Paediatric Societies

COMMERCIALLY PROCESSED HUMAN MILK AND HUMAN MILK-DERIVED PRODUCTS CONTAIN A BROAD SPECTRUM OF HUMAN MILK OLIGOSACCHARIDES

human milk

human milk oligosaccharides

pasteurization

prematurity

Abstract

Abstract

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Keywords

human milk

human milk oligosaccharides

pasteurization

prematurity

Abstract

Background and Aims: We have previously shown that infants fed an exclusive human milk diet (EHMD) demonstrate a statistically significant reduction in necrotizing enterocolitis (NEC) and sepsis when compared to infants fed a cow-milk based diet. Breast milk contains many bioactive substances including human milk oligosaccharides (HMO). HMO may reduce NEC and sepsis by promoting colonization by commensal bacteria that out compete colonization by the pathogenic bacteria that underlie these diseases in VLBW infants. We sought to explore the impact of industrial processing on the concentration and spectrum of HMO present in human milk and a human milk derived-protein fortifier. Methods We used high performance anion exchange chromatography with pulsed amperometric detection with known HMO standards to determine the spectrum and concentration of HMO present after industrial processing of donor human milk and a human milk derived-human milk protein fortifier. A simplified HMO extraction method was developed to avoid the use of chemical reagents and solid phase extraction thereby reducing the artificial loss of HMO. Results: We identified a full spectrum of neutral and sialylated HMO in pasteurized donor human milk and human milk protein fortifier similar to native breast milk prior to processing. Conclusions: We have developed a method for detecting and quantifying diverse and naturally occurring HMO in industrially processed human milk and human milk-derived fortifier. We have demonstrated that industrially processed donor human milk products are a robust source of HMO. These data support that some industrial processing preserves the content and likely activity, of HMO in human milk-derived products.

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© Copyright 2019 Morressier GmbH.
All rights reserved.