The merits of Dental Pulp Stem Cells (DPSC) in regenerative medicine cannot be understated. Variable substrate film modulus and the presence of titanium nanoparticles have been demonstrated to induce favorable pathways for differentiation. This study aims to draw a relation between TiO2, templated biomineralization, and differentiation of DPSC. Si wafers were cut and coated with thin (TN) or thick (TK) films of polybutadiene (PB), with some samples further treated with 80 cycles of Atomic Layer Deposition of TiO2 at Brookhaven National Laboratories. All wafers were cultured with human DPSC, with data samples taken weekly. Early on, images taken with Confocal microscopy indicated that cell doubling time between the PBTK and PBTN groups was similar, indicating that the presence of TiO2 had no significant effect on cell proliferation. Similar actin density and length within each group, PBTK and PBTN, regardless of ALD presence, further corroborates the principle that a physiological presentation of the chemical effects of the titanium nanoparticles are minimal in the initial period. In later weeks of the study, biomineralization SEM data reveals that ALD samples, regardless of the thickness or thinness of polybutadiene layer, exhibited substantially more collagen banding than the non-ALD samples did. This expression indicates that TiO2 is a physiological impetuous for DPSCs for this favorable behavior. OCN Confocal imaging confirms such a notion, displaying a more evenly distributed framework for ALD cells. The results concluded from this study posit that an ALD coating of titanium nanoparticles on any polymeric substrate will allow for templated biomineralization. RT-PCR and Raman further characterize later-stage cells.

Determining physical and chemical effects of ALD TiO2 on DPSC differentiation and biomineralization in PB films

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.