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Effects of surface roughness of Ce-TZP Al2O3 on the morphology and function of human gingival fibroblasts

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EAO-2018

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Abstract

:While the epithelial and connective tissue attachments to the tooth has been well documented, the attachment of peri-implant soft tissues to dental implants remains uncertain. Recently, dental implants made of zirconia (Zr) have been developed and introduced as an alternative to titanium (Ti). Our previous study demonstrated the strength of osseointegration of Ce-TZP/Al2O3 was greater than that of acid etched Ti implant in a rat model. However, the attachment of Ce-TZP/Al2O3 to the soft-tissues remains unknown. :In order to evaluate the capability of Ce-TZP/Al2O3 to attach peri-implant soft tissue, this study assessed the attachment, proliferation, morphology, and extracellular matrix (ECM) synthesis of human gingival fibroblasts (HGF-1) on the Ce-TZP/Al2O3 and Ti surfaces with different grades of roughness. <Material and Methods>:We prepared three types of Ce-TZP/Al2O3 substrates with different grades of surface roughness (mirrored Zr, smooth Zr and rough Zr) as test surfaces, and two types of Ti ( machined and mirrored Ti) substrates as control. We observed the surface characteristics of all samples by SEM. Initial cell attachment and proliferative activities were evaluated after 3h, 24h, and 3days of cultivation on the disks. Cell morphologies were observed using immunofluorescent staining after 3h of incubation. We measured the typeⅠ and Ⅲ collagen synthesis on the HGF-1 disk cultures after 7 and 14 days. Expression of inflammatory cytokines, including TNF-α, IL-1 and IL-6 assessed after 30 minutes of cultivation in the HGF-1 cultures. :Early response of HGF-1 fibroblasts on mirrored Zr was significantly greater than that on Ti surfaces. The highest proliferative capacities were observed on the mirrored Zr compared with the other disks. Confocal microscopic images of HGF-1 staining revealed that fibroblasts on two types of Ti showed typical spindle-shapes, whereas those on the Zr disks had round cells including contractile ring-like structures and developed cytoskeletons. The amount of collagen deposited on mirrored Zr increased significantly compared with the other disks(p<0.05). There were no significant differences between Ti and Zr at cytokines on culture 30 minutes. :The HGF-1 on mirrored Zr surfaces exhibited more matured morphology and greater cellular viability and differentiation than those of the other surfaces, which might be their greater biocompatibility as demonstrated. These results indicate that the mirrored Ce-TZP/Al2O3 surfaces may have a higher affinity for peri-implant soft tissue than Ti and the Ce-TZP/Al2O3 with other roughness.

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© Copyright 2020 Morressier GmbH.
All rights reserved.