During skin aging the dermal thickness decrease dramatically, that lead to a visible reduction in the elasticity of the skin. Different cosmetic products use to increase derma thickness by rising water and glycosaminoglycan content or stimulation fibroblast function with special nutrients. In our study we offer to use inhibitors of enzyme that block degradation of extracellular matrix and can rise dermal thickness. Enzyme inhibitors etamsylate have antihyaluronidase action was used in our study. Methods. Experiments was done on the neonatal mice 14 day after birth, after back skin shaving in experimental mice was administered etamsylate topically twice a day during 5 days, in control group mice were treated with water solution mice topically twice a day during 5 days. The dermal thickness was evaluate with using routine histomorphological techniques and ImageJ program. Results. In the experimental group the dermal thickness increased up to 77% compare to control grope. Conclusions. In neonatal mice 14 day after birth mouse hair follicles almost simultaneously pass from hair stage anagen to catagen phase with decreasing dermal thickness. Administration of hyaluronidase inhibitor etamsylate avoid reducing dermal thickness in mice, that also need to test on human aging skin.

Enzyme inhibitor Etamsylate has an influence on dermal thickness.