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Sep 24, 2017

The 47th Annual Meeting of the European Society for Dermatological Research

Identification of the epidermal miRNome in psoriasis skin

;

Ankit Srivastava;

Lorenzo Pasquali;

Florian Meisgen;

Mona Ståhle;

Ning Xu Landén;

Andor Pivarcsi;

Enikö Sonkoly

keratinocyte

psoriasis

microrna

Abstract

Abstract

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Keywords

keratinocyte

psoriasis

microrna

Abstract

Identification of the epidermal miRNome in psoriasis skin Psoriasis is a common chronic inflammatory skin disease, in which chronic skin inflammation is thought to be a result of interactions between keratinocytes and the immune system. MicroRNAs (miRNAs) are short noncoding RNAs that regulate the expression of the majority of protein-coding genes and are thus potent regulators of cellular functions. We have previously identified miRNAs deregulated in psoriasis skin, which regulate keratinocyte and/or immune cell functions. Here, we analyzed the cell-specific miRNome in epidermal cells in psoriasis, by next-generation sequencing (RNAseq) of small RNAs in CD45- (non-immune) epidermal cells sorted from the lesional and non-lesional skin of psoriasis patients, and from healthy skin of control subjects. This approach enabled us to study the keratinocyte-specific miRNome in psoriasis, which in previous studies utilizing full-depth biopsies, may have been masked by expression in other cell types. Our results revealed differential expression of 104 miRNAs in the epidermal cells in psoriasis lesions, including known and novel miRNAs that have not been previously associated with psoriasis. MiR-149 was identified as one of the significantly downregulated miRNAs in psoriatic epidermal cells. qPCR analysis confirmed its downregulation in psoriatic lesional epidermal cells as compared to those from non-lesional or normal skin. Notably, miR-149 was previously shown to have anti-inflammatory functions in other cell types. Taken together, our results identified the miRNA landscape in epidermal non-immune cells, and identified known and novel miRNAs previously not associated with psoriasis. These results can provide a basis for further functional studies of miRNAs in keratinocytes, which can identify miRNAs that can be targeted for topical therapy.

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© Copyright 2019 Morressier GmbH.
All rights reserved.