Introduction: In order to overcome Clorhexidine 0,12% adverse effects, a mouthwash contaning Casearis sylvestris was developed. Objectives: The purpose of this study was to evaluate the influence of a mouthwash containing Casearia sylvestris on color alteration and surface roughness of teeth previously submitted to bleaching or in contact with acid drinks. Material and Methods: Thirty cylindrical bovine teeth samples were divided in 3 groups, according to the solution evaluated: A) distilled water, B) Chlorhexidine 0,12% and C) a mouthwash containing Casearia sylvestris. Each group was divided in subgroups, according to the categories (n=10): 1) No treatment (control group), 2) bleached teeth and 3) teeth immersed in an acid drink (pH 2,4). The bleaching procedure was performed with carbamide peroxide 16% during 6 hours by 8 days, totalizing 48 hours of immersion. Whereas the acid drink immersion was performed once in a day during 2 minutes each immersion, for 6 days. Afterward, the samples were immersed into a bowl containing the respective solutions by 2 minutes, three times per day, during 7 days. The color and surface roughness were evaluated before the first immersion on the solutions and after 7 days of immersion. In addition, a qualitative analysis was performed by scanning electron microscopy. Results: Data were statistically analyzed by two-way analysis of variance (ANOVA) and Tukey`s test. Teeth submitted to bleaching or immersed in acid drinks presented a higher color alteration in comparison to the control group. Clorhexidine 0,12% (B1-0.21±0.08, B2-0.84±0.08, B3-0.69±0.08) and the mouthwash containing Casearia sylvestris (C1-0.53±0.08,C2-0.71±0.08, C3-0.74±0.08) did not provide a statistically higher color alteration in comparison to the distilled water (A1-0.0±0.08; A2-0.72±0.08, A3-0.80±0.08). The surface roughness was not altered by the solutions and treatment provided. Conclusion: The mouthwash containing Casearis sylvestris can be safely used on teeth previously submitted to bleaching or immersed in acid drinks during the period evaluated in this study.
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