and 1 other(s)
Background:A novel and highly efficient method for integration stripe patterns into pore walls of 3-D porous hydroxyapatite scaffolds (HAW) was developed using sugar spheres as porogens. In our previous research,the HAW were appropriate for the adhesion of cells and the formation of capillaries, coupled with excellent osteoconductive and osteoinductive potential. Numerous blood vessels and ectopic bone formation were observed when implanting HAW into the dorsal muscle of dogs . Aim/Hypothesis:To evaluate the osseointegration of dental implant at different time point inserted in HAW,which prior to be inserted for repairing canine segmental mandibular bone defects three months ago. Materials and Methods:Eight adults and healthy beagle dogs were selected and divided into two groups randomdly,bilateral mandibular premolars were extracted and two cylindrical bone defects of 8 mm in diameter and 10 mm in height were created.The HAW was inserted in the one side bone defect as test group,while autologous bone was used to fill the other side as control group.Three months later,a diameter of 4.0mm * 10mm Dentium implants were embeded into the middle of repair area. Four weeks and twelve weeks after implantation,imaging,histological and biomechanical methods were taken to evaluate the osseointegration of dental implant at 4 weeks and 12 weeks respectively. Resultats :A well implant-osseous healing was identified in both test and control group by X -ray and CBCT at 12 weeks after implantation, while the control group showed a higher neogenetic bone density around the implant. Micro-CT indicated a significant higher density difference in control group compared to the test one at four weeks (P<0.05),while the discrepancy disappeared at 12 weeks (P>0.05),which suggested similar conjunction were formed between the implant and the autologous bone as well as the HAW. Histologically,at four weeks both groups showed new bone formation, and lots of mature bone trabecula around implant thread at twelve weeks, simultaneously having detected Harvard tubule. No significant mechanical-strength difference was found in mandible between the experimental group and control group(P>0.05). Conclusions:The HAW in vivo alone could induce new bone formation,thus might be a favorable remediation to repair canine segmental bone defects.Moreover,they could achieve fine osseointegration between implants and HAW,especially when it comes to 12 weeks.
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