and 7 other(s)
Background The development of an antibacterial titanium surface is of great interest to prevent peri-implant infections. Staphylococcus aureus is frequently associated with orthopedic infections, and were found in 55% of the peri‐implant lesions. Our group has extensively documented the antibacterial effects of silver tungstate (Ag2WO4) microcrystals. Aim/Hypothesis This study aimed to evaluate a titanium coating with Ag2WO4 microcrystals to prevent Staphylococcus aureus adhesion. Materials and methods Ag2WO4 microcrystals were synthesized by co-precipitation method under vigorous magnetic stirring at 90 °C, for 10 min. Thereafter, the microcrystals were deposited onto titanium surfaces by spin-coating method, and the antibacterial activity against S. aureus ATCC 33591 was evaluated. The samples were coated with up to 3 layers of Ag2WO4 microcrystals. The microorganism was cultured in tryptic soy broth (TSB) culture medium until it reached the mid-log phase of growth. Then, the inoculum was standardized at a final concentration of 108 cells per mL and then seeded onto titanium surfaces for 90 minutes to assess the initial cell adhesion. Thereafter, the specimens were washed with phosphate-buffered saline solution (PBS) and prepared for scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) analysis. Results SEM micrographs showed significant reduction of adhered cells in Ag2WO4 coated samples compared to uncoated ones. Data obtained from CLSM images corroborate with SEM images. The absence of cells labeled with fluorides Syto 9 (green - live cells) and propidium iodide (red – dead cells) indicate that titanium coated Ag2WO4 microcrystals reduced S. aureus cell adhesion. The Ag2WO4 treatment decreased S. aureus adhesion accordingly to the number os coatings performed onto titanium surface. Conclusion and clinical implications The coating containing Ag2WO4 reduced the adhesion of S. aureus on the surface of titanium. After more studies, this coating may be used on titanium surfaces in general, in order to reduce peri-implants infections.
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