Background and Goal of Study: Cardiomyocyte death induced by ischemia/reperfusion injury is an important contributor for myocardial dysfunction. Propofol has been identified that it has a protective effect after myocardial ischemia/reperfusion injury through various experimental methods. We also reported that propofol has myocardial protective effect at the regional ischemia/reperfusion injury in vivo rat model. We presumed that the myocardial protective effect of propofol would be related with lipid emulsion which is used for solvent of propofol. In this study, we investigated that lipid emulsion modulates hydrogen peroxide (H2O2)-induced apoptotic cell death in H9c2 rat cardiomyoblast cells.Materials and Methods: H9c2 cells were treated with H2O2 for oxidative stress, and we examine the effect of lipid emulsion on oxidative stress-induced cell death. The effect on the cell viability of H9c2 cells was evaluated by using MTT assay, and Trypan blue. Protein expression of caspase 3, Bcl/2 (B-cell lymphoma 2), Bax (BCL2 Associated X Protein) was measured using the western blot analysis.Results and Discussion: Our results showed that H2O2 induced apoptotic cell death in H9c2 cells, which was associated with decreasing the level of Bcl2. However, decrease of these proteins was restored when pretreated with lipid emulsion and followed with the increase of Bcl2. Cleaved Caspase-3 expression was increased by H2O2 oxidative stress, and cleaved Caspase-3 expression was decreased by treatment of lipid emulsion.Conclusion(s): Our results demonstrate that oxidative stress promotes apoptotic cell death in H9c2 cells, and lipid emulsion regulates oxidative stress induced apoptotic cell death in myocardiocytes H9c2. These results suggest that lipid emulsion may be implicating in the myocardial protective effect against cardiac ischemic reperfusion injury.References: 1. Jin YC, Kim W, Ha YM, Shin IW, Sohn JT, Kim HJ, Seo HG, Lee JH, Chang KC. Propofol limits rat myocardial ischemia and reperfusion injury with an associated reduction in apoptotic cell death in vivo.Vascul Pharmacol. 2009:50(1-2):71-7.2. Shin IW, Jang IS, Lee SH, Baik JS, Park KE, Sohn JT, Lee HK, Chung YK. Propofol has delayed myocardial protective effects after a regional ischemia/reperfusion injury in an in vivo rat heart model. Korean J Anesthesiol.

Lipid emulsion attenuates H2O2-induced apoptotic cell death in H9c2 rat cardiomyoblast cells