A clinical implementation of in house produced acellular dermal matrix (ADM) inhabited by in vitro cultured cells – case series.
Wojciech Łabuś1, Justyna Glik1,3, Mariusz Maj1, Agnieszka Klama-Baryła1, Diana Kitala1, Małgorzata Kraut1, Mariusz Nowak1, Marek Kawecki1,2

1 dr Stanislaw Sakiel Centre for Burn Treatment in Siemianowice Slaskie, Poland
2 Department of Health Sciences, Technical-Humanistic Academy in Bielsko Biala, Poland
3 Unit for Chronic Wound Treatment Organization, Division of Nursery,  School of Health Care, Medical University of Silesia, Poland

Abstract

Objectives 
An acellular dermal matrix (ADM) can be repopulated de novo by autologous or allogeneic skin cells. The goal was to evaluate a new burn treatment methods based on usage of self produced human biovital skin substitute.
Material and methods
4 patients were selected for the study. All patients were thermally burned and treated with allogeneic, biostatic skin grafts and in-house produced ADMs revitalized with autologous or allogeneic fibroblasts and keratinocites or allogeneic amnion derived mesenchymal stem cells. Allogeneic, biostatic skin grafts were used as a control. Cells were suspended in autologous platelet leukocyte rich gel PLRG or in buffered saline PBS. Photographic documentation was executed as well as histological and microbial examination.
Results
Photographic and histological images depicted a burn wounds in the appropriate healing progress. 
Discussion / Conclusions
The presented method for burn treatment may be the optimal method for burn wound therapy. However, it requires increased number of samples to perform statistical analysis of the results.