A comparison of features of transgeneic porcine skin wound dressings and biostatic allogeneic human skin transplants
PhD Klama-Baryła, Agnieszka, Centrum Leczenia Oparzeń im. dr. S. Sakiela, Siemianowice Śląskie, Poland.
Objectives: The aim of the study was to clinically evaluate a biostatic skin allografts, transgeneic porcine xenografts and revitalized porcine acellular dermal matrix (PADM) in a treatment of burn wounds.
Materials and methods: Transgeneic porcine skin grafts and human skin allografts were used in the study. Human keratynocites and fibroblasts were used for PADM revitalization. The decellurization methods included: 3% Triton-X, 0,1% SDS, 0.5% tripsin, 2,4 U/ml of dispase II, 85% glicerol and 15% glicerol. Mechanic and biological features of grafts were evaluated.
Results: A porcine skin preserved by use of 15% glicerol is much more clinically effective than 85% glicerol (low elasticity, high hardness and displeasing visual features). Porcine skin incubated in 85% glicerol has a negative effect on live skin cell cultivation and ability to split, which is not the case with 15% glicerol soaked porcine skin. As the best decellurization method the trypsinization was chosen. Additionally, trypsinized PADM showed to be the best scaffold for human keratinocytes and fibroblasts in vitro culture. Fibroblasts and keratinocytes cultured on porcine skin decelluralized by trypsination have proven to be the most vital ones, hinting at positive results of future transplants using these cells.
Discussion: The decellurization of porcine and human skin by trypsin has proven that both matrix are interchangeable in cases of insufficient supply of autologous human skin. 15% glicerization has proven to be the best method for conserving procine skin.
Presented work was financially supported by The National Centre for Research and Development (grant number INNOMED/I/17/NCBR/2014 as part of "INNOMED" entitled: "Development of Innovative Technologies for the use of Transgenic Pig Tissues for Biomedical Purposes”.Acronym "MEDPIG"

A comparison of features of transgeneic porcine skin wound dressings and biostatic allogeneic human skin transplants.