Evaluation of mitochondria-organelle peptides versus nano-sized cellular extracts in aesthetic dermatology: comparative case study.

Comparative case study aimed to analyze efficacy of two peptide formulas: F1 with molecular weight 1-10 kDa and F2: combination of 1-10 kDa with 45-65kDa. Twice a week injections performed 6 weeks on 10 volunteers for each formula spending 2 ml on upper face. All 20 volunteers completed protocol and showed improvement 45-50% on F1 and 60–75% on F2 in skin tightening and texture at end of treatment vs 35-45% and 50–60% at 3 months later respectively. Clinical improvement of wrinkles ranged from good to moderate in F1 and good in F-2. Starting from week 3 average roughness decreased by 14.1% and 19.7% on F1 vs to 27.6% and 34.5% on F2 at end of study and 3 months after. Total wrinkle height decreased by 23.4% and 17.3% on F1 and by 26.7% and 25% at end and 3 month later for F2. Collagen type I and type III showed highest on F1: 68.6% and 67.2% vs  F2  changes 69% and 69.4% accordingly (P≤0.05). F1 showed newly synthesized collagen 14.6%, 17.5% and 16.3% before, at end and post treatment compare to F2 which has more significant result 14.7%, 18% and 17.2% respectively (P≥0.05). Quantitative evaluation of type VII collagen revealed differences between F1 and F2: 10.2% vs 10.6% at baseline, 11.2% vs 12.6% at end and 10.9% vs 11.9% at 3 months post-treatment. Total dermal elastin on F2 and tropoelastin levels increased compare to F1: 14.2% vs 13.2% prior to treatment, 16.8% vs 14.2% levels at end and 15.2% vs 13.8% at 3 months later P≥0.05. Both formulations achieved good outcome on 4 weeks of treatment with high patient satisfaction confirmed by GAIS scale. In conclusion, minimally invasive mesotherapy with F1 and F2 demonstrated effectiveness supported by instrumental and clinical evaluations. Obtained results confirmed F2 statistically considerable outcomes on treatment and prolonged effect compare to F1. Clinically F2 appeared visible on periorbital area smoothing numerous fine lines whereas F1 revealed highly noticeable effect on forehead. In future clinical practice it is advisable to use both formulas on different areas in accordance to achieve maximum result.