Background: The outcome of an in vitro fertilization (IVF) cycle will be determined by several factors including oocyte quality. Inhibin-B has a predicting value for follicular development and a predictor of embryo quality. The objective of this study was to investigate the association between serum inhibin-B level with the number of picked-up oocyte, mature oocyte, and fertilization rate in IVF cycle and to determine their cut-off levels.

Materials and methods: An analytic cross-sectional study was conducted from September 2013 until August 2014 at Harapan Kita Mother and Chil­dren Hospital Jakarta. A total of 38 infertile women undergoing controlled ovarian stimulation for IVF cycles were measured for serum inhibin-B levels at basal, trigger, and ovum pick-up (OPU) times. The receiver operating characteristic analysis (ROC curve) was used to determined the cut off-level of serum inhibin-B to predict the number of picked-up oocyte (>3 oocytes), mature oocyte (>3 oocytes), and fertilization rate (>70%).

Results: The median level of inhibin-B during OPU was 196.31 pg/ml and it was significantly correlated with the number of picked-up oocyte (p=0.017), mature oocyte (p=0.005), and fertilization rate (p=0.021). Serum inhibin-B level during OPU ≥ 131.17 pg/ml was moderately accurate to predict the number of picked up oocyte of more than 3 oocytes (AUC 0.75, sensitivity 81.5%, specificity 72.7%) and the number of mature oocyte of more than 3 oocytes (AUC 0.77, sensitivity 84.0%, specificity 69.2%). Serum inhibin-B level during OPU ≥ 86.84 pg/ml was also moderately accurate to predict fertilization rate >70% (AUC 0.74, sensitivity 88.9%, specificity 63.6%).

Conclusions: Serum inhibin-B level during OPU was associated with the number of picked-up oocyte, mature oocyte, and fertilization rate in IVF cycle. The cut-off levels were moderately accurate to predict several parameters of oocyte quality.

Serum Inhibin-B Level During Ovum Pick-Up As A Biochemical Predictor For Oocyte Quality In Ivf Cycle