Ischemic stroke is known to reach 80% of all strokes and is the second most common cause of death worldwide. Stroke pathology in rodent models are not similar to human one and pathological events at acute stage of stroke are not known. In order to overcome these limitations, a non-human primate stroke models were generated through seven Cynomolgus macaques to middle cerebral artery occlusion (MCAO) and samples were collected at D-8 (8 days before MCAO), Pre-recanalization (Post-occlusion), Post-recanalization (Post-reperfusion), PR-1day, PR-7day, PR -1month (1day, 7day, 1month after post-recanalization). To analyze protein expression by time, cytokine/chemokine protein array were performed. Several proteins such as growth factor, interleukins and chemokine showed the significant changes at acute stage by time dependent manner. In the observed proteins, CXCL1, GM-CSF, IL13, IL21 proteins changed after Post-recanalization. In conclusion, these results suggested that the alteration of these protein may indicate the pathological events at acute stage of ischemic stroke.

The analyses of biomarkers at acute stage in ischemic stroke model using non-human primates