Outbreaks of Gonipterus platensis (Coleoptera: Curculionidae) in Eucalyptus plantations have occurred in the States of São Paulo and Paraná, Brazil, in the last years, due to low parasitism rates of the egg parasitoid Anaphes nitens (Hymenoptera: Mymaridae). Symbiont bacteria can act in different ways on hosts, influencing the parasitism’s efficiency. However, there are no records of symbionts in A. nitens. This work aims to identify symbiotic bacteria in different parasitoid populations and eggs of their host. Adults of six populations of A. nitens (five form São Paulo and one from Espírito Santo state) and eggs from a laboratory population of G. platensis were evaluated by PCR using specific primers for identification of nine different genera of cellular endosymbionts. The purified PCR products were sequencied and the sequences obtained were compared to GenBank database. The bacterium Serratia grimesii was identified in the eggs of G. platensis, it was already reported in midgut of other Coleoptera. All populations of A. nitens had presence of cellular endosymbiont Rickettsia belli and bacteria Erwinia amylovora, Yersinia massiliensis and S. grimesii. Bacteria E. amylovora and Y. massiliensis probably act as intestinal symbionts for the host. S. grimesii, found in the parasitoid and host, probably is acquired by horizontal transmission from G. platensis. Studies to verify the bacteria quantitative aspects and the effects on A. nitens biological parameters are ongoing.

07 - Detection of symbiotic bacteria in Anaphes nitens (Hymenoptera: Mymaridae) and Gonipterus platensis (Coleoptera: Curculionidae) eggs