Background and aim: In this study, part of a larger one, we studied the antinociceptive action of the fixed combination of cyclobenzaprine hydrochloride, lidocaine and piroxicam as a topical preparation, in mice.
Methods: The study was conducted on groups of 10 Swiss male mice weighing 20.00-30.00g, treated with applications of 0.1 ml gel on the hind-paws at 0 and 60 minutes with: gel base, lidocaine 2%, piroxicam 0.5%, cyclobenzaprine 0.5% and their combination. 60 minutes after the application the animals were tested at 52.5 ± 0.2 ° C (cut off = 30s) at 0, 15, 30, 60, 90 minutes. The MPE (maximum possible effect of antinociception) % of the single substances and their combination has been calculated compared with the control group; for the statistical analysis was used the T-test.
All experiments have been made in accordance with the EU bioethical rules and regulations (EU Directive 63/2010). 
Results: Antinociceptive effects were demonstrated for all treated groups at various times of testing. For the fixed combination, the antinociceptive effect was demonstrated for the T0-T90 interval (T0: 80.6%, p <0.0001, T15: 39.5% p = 0.023, T30: 43.9%, p = 0.0068, T60 : 50.1% p = 0.019; T90: 43.1%, p = 0.0145).
Conclusion: The statistical significance of the data shows a persistent antinociceptive (for at least 90 minutes) effect of the combination in the hot-plate test, compared to the control groups.
Acknowledgments: The paper presents partial results from a larger research grant funded by Fiterman Pharma Iasi, Romania (grant 6451 / 19.05.2016)

EVALUATION OF THE ANALGESIC ACTION OF THE COMBINATION CYCLOBENZAPRINE HYDROCHLORIDE, LIDOCAINE AND PIROXICAM IN MICE USING THE HOT PLATE TEST