Stabilization and activity of biomolecules such as proteins and nucleic acids is very crucial in applications such as biosensing and drug delivery. In this presentation, the in-situ synthesis and stabilization of enzyme-modified gold nanoparticles without the use of activators or organic stabilizers will be discussed. The enzyme acted not only as a reducing agent of the gold salt precursor, but also as a stabilizing agent of the resulting gold nanoparticles. The enzyme/nanoparticle conjugates were coated with poly(ethyleneimine) (PEI), a well-known cationic polymer used for transfection purposes. Further, the activity of the resulting PEI-coated enzyme/nanoparticles conjugates was evaluated. The activity of these nanoparticle conjugates were compared with those prepared from the well-known method of synthesizing gold nanoparticles via citrate reduction followed by stabilization with organic molecules (mercaptoundecanoic acid (MUA)). The residual activity of the enzyme was much longer for the PEI-enzyme/nanoparticle conjugates than for the PEI-enzyme/MUA nanoparticle conjugates. The nanoconjugates were characterized by Dynamic Light Scattering, UV-Vis spectroscopy, IR spectroscopy and Transmission Electron Microscopy (TEM).

Enzyme-based <i>in situ</i> synthesis, stabilization and activity of gold nanoparticles for biological applications

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.

Enzyme-based in situ synthesis, stabilization and activity of gold nanoparticles for biological applications