Although concerns of fine particulate air pollution are growing, our understanding of the health implications of inhaled nanoparticle aerosols is still incomplete due to lack of real-time measurement of disease progression at cellular and molecular levels. To mimic the complex composition, physiological function and cell diversity of human lungs, we built a 3-dimensional human lung model cultured at the air-liquid interface. We aim to understand the interaction between human lung models and nanoparticle aerosols through investigating how the surface chemistry and doses of gold nanospheres influence human small airway epithelium differentiation. To mimic human inhalation, gold nanosphere aerosols were delivered to cocultures of primary human small airway epithelial and pulmonary artery endothelial cells through nebulization. Our lung models were exposed to human realistic ambient and worst-case exposure with tissue dose fluxes 5.6 × 106 and 4.6 × 108 nanospheres/cm2, respectively. We monitored the long-term progression of inflammatory responses present in the cocultures from the 1st day of gold nanosphere aerosol exposure to the end of 21st day until epithelium differentiation. Although all human lung models successfully differentiated, we observed more than three-fold increases in interleukin 6 on the 21st day, showing the possibility of chronic inflammation in the cocultures exposed to gold nanosphere aerosols regardless of nanoparticle surface chemistry and doses.

Impact of gold nanoparticle aerosols on human small airway epithelium differentiation

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.