Cellulose is a major component of plant primary cell wall, which is a highly hydrated system of polysaccharides responsible for the mechanical properties of plant tissues during growth. Much of the characterization of plant cellulose has been conducted in the dried state, mainly due to the instrumental limitations of various techniques. For example, in the case of X-ray scattering, the amorphous scattering of water often dominates data. Nevertheless, drying of the sample may lead to artifacts that are not representative of the highly hydrated plant primary cell wall. Thus, we have examined the crystalline parameters of hydrated plant primary cell wall using Grazing-Incidence Wide-Angle X-Ray Scattering (GIWAXS).  GIWAXS enables high signal-to-noise from thin, wet tissues due to enhanced scattering near the critical angle of cellulose. Scattering experiments on onion epidermis under humid conditions reveal Angstrom-level changes in the structure of semi-crystalline cellulose microfibrils. The removal of water around the microfibril was found to interfere with (110/1-10) lattice packing, as indicated by changes in peak intensities and lattice spacings.<br/>

Effects of dehydration on plant primary cell wall cellulose from grazing-incidence wide angle X-ray scattering

Macromolecular Chemistry: The Second Century

[Author Benefits](https://scimeetings.acs.org/?utm_source=pubs_content_marketing&utm_medium=website&utm_campaign=0320_MCF_NPI_Launch_Spring_Homepage&ref=pubs_content_marketing)

[How to use SciMeetings](https://storage.googleapis.com/dl.morressier.com/acs/SciMeetings-ACSSpring2020-HowToGuide_M3020-Final.pdf)

[Frequently Asked Questions](https://storage.googleapis.com/dl.morressier.com/acs/SciMtng_ACSNMSp2020_FAQ_M3120_Final.pdf)

ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.