<br/><br/>Gold nanoparticles (AuNPs) have been exploited in the various domains of science such as drug deliver, biosensing, immunoassay and environmental sensors, due to their optical properties and intriguing surface chemistry. Different scientific procedures have been used to effectively immobilize antibodies onto gold nanoparticles. Although acceptable outcomes have been achieved in the immobilization of antibodies onto gold nanoparticles, the sensitivity of these immobilized antibodies to target antigen or binding sites is limited due to improper orientation of the antibodies. There is some evidence that the surface charge of antibodies is responsible for controlling the orientation upon adsorption to gold nanoparticles. Antibodies have ubiquitous lysine residues which are protonated at physiological pH contributing to the total surface charge of the antibody. Chemical modification of antibodies by reacting with NHS acrylic acid, acetylate the lysine residues consequently controlling the surface charge of the antibodies and potentially impacting the orientation. In this proceeding, novel analytical techniques are being utilized to directionally adsorb charge modified antibodies onto citrate capped gold nanoparticles to increase the amount of exposed active site. Dynamic light scattering, fluorescence, nanoparticle tracking analysis and other analytical strategies have been used to study the adsorption dynamics as well as the orientation of these charged modified antibodies on gold nanoparticles. These fundamental investigations to elucidate the mechanism of protein-AuNP adsorption will lead to optimized bioconjugates that are necessary to realize the full potential of AuNP-enabled bio nanotechnologies.

Adsorption behavior of chemically/charged modified antibody on gold nanoparticles

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.