Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) has a number of real world applications, including forensics, pharmaceuticals, and medical diagnosis. SALDI-MS provides straightforward sample preparation and quick results. Non-functionalized transition metal oxide (TMO) nanoparticles make ideal surfaces for the SALDI-MS analysis for a variety of reasons. TMO nanoparticles do not cause matrix interference in the low mass range because they are inert, allowing for the detection of both large and small molecules. They can efficiently adsorb UV light and have a high surface area to volume ratio. These qualities make adsorption of a wide range of analytes efficient. Previous experiments have shown iron (II,III) oxide (Fe3O4) and cobalt (II,III) oxide (Co3O4) nanoparticles to be suitable for the SALDI-MS analysis of biomolecules. Herein we show TMO nanoparticles are also suitable SALDI surfaces for the analysis of complex mixtures and real world samples. A human serum sample and commercial grade tonic water were analyzed via SALDI-MS using both Fe3O4 and Co3O4 nanoparticles. In addition, human serum samples spiked with adenosine and tonic water samples spiked with quinine were analyzed using via SALDI-MS. Signal-to-noise (S/N) ratios and signal intensities for adenosine and quinine in the LDI method were calculated and compared. Preliminary results indicate that SALDI-MS analysis of complex mixtures using Fe3O4 and Co3O4 nanoparticles yields higher signal intensities than LDI.

SALDI-MS analysis of real world samples utilizing transition metal oxide nanoparticles as surfaces

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.