Vitamin C (VC) is a natural antioxidant and plays an important role in various physiological processes such as defense against cellular oxidation, quenching of free radicals, and prevention of cardiovascular disease and cancer. Several studies have also shown that vitamin C can significantly reduce the concentration of uric acid in blood by enhancing excretion of uric acid, a main compound associated with gouty arthritis. Thus it is important to simultaneously determine vitamin C, uric acid and a biomarker creatinine in human fluid samples. Many analytical methods such as spectrophotometry, voltammetry, GC, CE, and HPLC have been developed for the determination of these analytes in human fluids. Due to the complex matrices with numerous nonspecific interferences for the determination in biological samples by other techniques, HPLC has gained popularity. As VC, uric acid and creatinine all are very polar small compound, it is difficult to be retained in conventional RP-HPLC and separated from void volume. A rapid and sensitive hydrophilic interaction chromatography (HILIC) method has been developed here for the determination of VC, uric acid and creatinine simultaneously in human fluids. Human fluid samples were deproteinated in the HILIC mobile phase, and then centrifuged, filtered, and separated on a Waters Spherisoro S<sub>5</sub>NH<sub>2</sub>column fitted with a C18 guard column using an isocratic elution program consisting of a 50% acetonitrile and 50% aqueous phosphate buffer solution. The UV detection was made at the wavelength of 205 nm. Three analytes had a good retention on the amino column and were well separated from other components in human fluid samples. The developed HILIC technique has been successfully applied to examine the absorption and metabolism of VC in human fluids and VC induced uricosuria.

Simultaneous determination of vitamin C, uric acid and creatinine in human fluids and vitamin C-induced uricosuria by HILIC

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.