In the United States, loss of hearing impacts approximately 48.1 million people. The cumulative effects of noise are experienced in every area of society whether occupational, environmental, or through aging. Previous work has reported changes in dopamine receptor gene expression following acoustic trauma, suggesting a possible role of dopamine in auditory processing. This conclusion is supported by recent data that showed patients suffering from Parkinson’s disease (a condition associated with dopamine depletion) exhibit deficits in auditory processing. Thus, the present work focuses on the role of dopamine neurotransmission within the central auditory pathway and how it is impacted by noise exposure. Characterizing the complexities of neurotransmission requires elegant methods of inquiry, regarding both the neurotransmitter and neuron physiology. Fast scan cyclic voltammetry (FSCV) with carbon fiber microelectrodes is uniquely well-suited for real time neurochemical measurement because it has the speed, selectivity, sensitivity, and the spatial resolution needed for such measurements. Immunoassays on the other hand provides information about the neural protein receptors distribution and levels. In this work dopamine neurotransmission release and uptakes events are measured and characterized using FSCV in the inferior colliculus in vivo and in brain slices by both electrical and sound stimulation. Western blot and immunocytochemistry are utilized to assess the impact of noise on dopamine receptor levels and distribution respectively. The combination of FSCV with immunoassays allows for a more comprehensive understanding of dopamine’s role in the central auditory pathway.<br/>

Electroanalytical tools and molecular-based assays to measure the impact of noise on dopamine neurotransmission in the central auditory pathway

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.