New technologies utilizing nontraditional antibiotic mechanisms are urgently needed to combat the increasingly common appearance of multi-drug resistant bacteria. Drug resistant bacteria is a worldwide problem as the number of drug resistant pathogens grows with each year. The World Health Organization developed the Global Action Plan on Antimicrobial Resistance (AMR) in 2015 to control the spread and impact of antimicrobial resistance. In addition, the economic cost of AMR to be approximately 3.1% of global output gross domestic product. To put this in prospective, the cost of cancer represents 1.5% of global GDP. This work explores the feasibility of using magnetically hyperthermia to selectively kill enterotoxigenic Escherichia coli strain K99 (EC K99) as a model for antibiotic resistant bacteria.<br/>Click ready magnetic nanoparticles were synthesized and functionalized with bacteria-specific glycoconjugate for adherence to EC K99. When mixtures containing both EC K99 and the GM3-MNPs were exposed to alternate magnetic fields (31 KA/m, 207 KHz), a clinically relevant 3-log reduction in colony forming units (CFUs) of EC K99 was achieved in 120 minutes. Bacterial selectivity of the treatment was shown using a mixed culture experiment including both recepter positive EC K99 and recepter negative EC O157. Targeted cell death of the EC K99 was seen after treatment of the mixed culture with minimal damage to EC O157. Cell death of EC K99 was further supported by the intracellular adenosine triphosphate (ATP) levels which were considerably reduced when incubated with GM3-MNPs and treated with MagMED. These results suggest that GM3-MNPs induced glycoconjugate targeting along with MagMED can be potentially used as a targeted nontraditional antibiotic treatment platform to inactivate/kill bacterial pathogens, with minimal impact on normal microflora and the affected body region/tissue.

Glycoconjugate-functionalized magnetic nanoparticles: Tool for selective killing of targeted bacteria via magnetically hyperthermia

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.