Super-resolution imaging is a useful tool to gain information not only about the shape and orientation of metallic nanoparticles but also how fluorescent biological ligands bind to the surface. One major problem with the technique is that the reconstructed image is consistently smaller than the expected size of the particle. It is hypothesized that this error in localization may be due to coupling between the plasmon mode of the nanoparticle and the fluorophore. The degree to which they are coupled can be investigated by studying the orientation of the fluorescent emitter on the rod by defocused imaging.<br/>

Using defocused images to investigate fluorophore-plasmon coupling interactions and their relation to localization accuracy in super-resolution imaging

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.