Ethylene is a key modulator in the ripening of climacteric fruits and is commercially indispensable in the synthesis of common plastics such as polyethylene. Despite its multifaceted importance in society, there is a dearth of methodologies available to detect ethylene, with most techniques reliant upon complex instrumentation with limited potential within complex biological systems. Alternatively, small molecule probes grant insight into cellular systems and are used extensively in molecular biology. As such, we recently developed a small molecule probe that makes use of ruthenium catalyzed olefin metathesis to selectively release a fluorescent molecule in the presence of ethylene. Strategies for modifying this system for application as an artificial metalloenzyme will be presented. This system allows for not only the selective detection of ethylene within living systems, but also the localization of a metalloenzyme for location specific detection of ethylene in cells.

Construction of an ethylene detecting metalloenzyme in <i>E. Coli</i> using a novel ruthenium-based probe

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.

Construction of an ethylene detecting metalloenzyme in E. Coli using a novel ruthenium-based probe