DNA exists primarily as a duplex. However, single-stranded DNA (ssDNA) is temporarily exposed in several cellular processes, such as DNA replication and transcription. ssDNA can form its self-folding (secondary) structure by sequences of DNA and the secondary structure of ssDNA affects the cellular processes. For example, the stable secondary structure of ssDNA can cause DNA mutations, both extension and contraction. Therefore, discovering selective binders that stabilize the secondary structure of ssDNA is important as a tool for understanding biological events more and as a potential treatment for diseases caused by the mutated DNA. The target DNA strand (CCG)7, which builds the stable secondary structure, was assayed with selected 80 compounds which have more than 80 % of similarity of the known C-C mismatched base pair binder. The six hits were tested for binding affinity and selectivity toward the secondary structure of (CCG)7 repeats. The stabilization of the secondary structure of d(CGG)7 repeats by the hit compounds was examined by measuring the Tm.

Developing small molecules selectively targeting the sequence-based secondary structure of ssDNA, CCG repeats and their effect in biological processes

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.