Riboswitches are untranslated regions within bacterial mRNA that bind specific intracellular metabolites. The xpt riboswitch is a guanine responsive riboswitch involved in purine biosynthesis and transport pathways. This riboswitch inhibits purine gene expression in the presence of bound guanine. When guanine is bound, the mRNA changes its three-dimensional structure that subsequently inhibits transcription of the xpt purine synthesis gene. In this work, we have synthesized purine nucleobase and nucleoside analogs in an effort to improve the binding affinity and solubility of compounds in our molecular assays. Our biochemical and cellular analysis looks at the binding and gene inhibition by the synthesized analogs in comparison to parent compounds.

Synthesis and evaluation of purine analogs that target Xpt riboswitch gene

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.