Lipid bilayer is a fundamental structure of cell membranes, and lateral and transversal distribution of lipid molecules are key factors during the transportation of materials and signals through the cell membrane. We have studied lipid bilayer membranes on graphene oxide (GO). GO has a unique fluorescence quenching property, and applied to biosensing recently. We have reported that less-fluid components localized on GO in multi-component lipid bilayer membranes on a SiO2/Si substrate partly covered with GO. In this study, we formed a tethered-type lipid bilayer on a chemically modified graphene oxide (GO), and investigated the localization effect of GO and the lateral diffusion in the tethered bilayer.<br/>GO was prepared by the chemical exfoliation of graphite particles, and spin-coated on a thermally oxidized SiO2/Si substrate. The GO/SiO2/Si substrate was treated with 1-pyrenebutiric acid N-hydrosuccinimide ester, followed by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride, and streptavidin. Atomic force microscope topographies showed that the GO flakes were covered with streptavidin molecules, which were observed as ~3 nm high protrusions, while bare SiO2 regions were kept flat.<br/>We prepared a planar lipid bilayer membrane comprising of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), biotin-N-1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (biotin-DPPE) and lissamine rhodamine B-N-DPPE (Rb-DPPE) (100:1:0.5) by the vesicle fusion method. AFM topographies showed flat morphology on the GO flake surface, and shape of streptavidin molecules were not observed. The GO flake region was higher than the surrounding lipid bilayer membrane on the bare SiO2 region by 7.1 nm. It corresponds to a thickness of GO (~1.5 nm thick) modified with streptavidin (~6 nm in diameter). A lipid bilayer consisting of DOPC, poly(ethylene) glycol-DPPE (PEG-DPPE) and biotin-DPPE (97.5:2.5:1) showed that the localization effect of GO did not appear on the streptavidin-modified GO, while PEG-DPPE domains were localized on non-modified GO. We conclude that a planar tethered-type lipid bilayer formed on the streptavidin-modified GO, and streptavidin worked as a spacer between the lipid bilayer and GO. We also describe the fluorescence quenching efficiency of lipid bilayers on GO with and without streptavidin modification.

Tethered-type lipid bilayer membranes on graphene oxide

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.