Helicobacter pylori and Campylobacter jejuni, which are known to be helically shaped Gram-negative bacteria, colonize the human mucus layer of the gastrointestinal tract and cause various gastrointestinal diseases such as chronic gastritis, peptic ulcer, gastroenteritis and gastric adenocarcinoma in humans. In the pathogenesis and gut colonization, the helical shape of H. pylori and C. jejuni is believed to be a critical factor in their motility improvement. Recently, a series of genes associated with maintaining the helical cell shape in these bacteria have been identified, most of which have been found to encode for either endo- or exoproteases that act on the peptide chains of peptidoglycan. These proteases are referred to as cell shape determining (Csd) proteins or peptidoglycan peptidase (Pgp). According to recent studies, inhibition of proteins responsible for the helical cell morphology could be useful in interference with the bacterial virulence and lifestyle, hence a very attractive therapeutic target. However, their catalytic processes and substrate recognition are largely unknown. In this study, we describe the synthesis of two substrates for Pgp3, one of the Csd proteins, and the analysis of the Pgp3-substrate complex structures of the active site of Pgp3 in high resolution.

Substrate synthesis and structural analysis of peptidoglycan peptidase3: Bacterial cell shape determining metallopeptidase

Macromolecular Chemistry: The Second Century

[Author Benefits](https://scimeetings.acs.org/?utm_source=pubs_content_marketing&utm_medium=website&utm_campaign=0320_MCF_NPI_Launch_Spring_Homepage&ref=pubs_content_marketing)

[How to use SciMeetings](https://storage.googleapis.com/dl.morressier.com/acs/SciMeetings-ACSSpring2020-HowToGuide_M3020-Final.pdf)

[Frequently Asked Questions](https://storage.googleapis.com/dl.morressier.com/acs/SciMtng_ACSNMSp2020_FAQ_M3120_Final.pdf)

ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.