Fluorescent imaging of fibrin could enable highly sensitive and noninvasive molecular imaging of thrombosis syndromes in vivo. The goal of this study was to develop a novel and more green synthesis of a peptide-based fibrin-targeted imaging agent that enables high-resolution near-infrared fluorescence (NIRF) imaging of deep vein thrombosis. The linear fibrin-targeted peptide was conjugated to a near-infrared fluorophore Cy7, termed FTP11-Cy7. The peptide was then subjected to intra-cyclization by formation of disulfides by air oxidation of thiols of cysteine under sonication. The NIRF peptide is based on a fibrin-specific imaging agent that has completed Phase II clinical magnetic resonance imaging trials. In vitro binding of FTP11-Cy7 to human plasma clots was assessed by using fluorescence reflectance imaging. Intravital fluorescence microscopy and noninvasive fluorescence molecular tomography-computed tomography were also performed. The fibrin-targeted NIRF agent FTP11-Cy7, synthesized using novel method, was shown to avidly and specifically bind human and murine thrombi, and enable sensitive, multimodal intravital and noninvasive NIRF molecular imaging detection of acute and subacute murine DVT.

Fibrin-targeted fluorescent probes for the molecular imaging of thrombi

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.