The concentration of nutrients influences cell growth, among which glucose is of the utmost importance as it is the main nutrient used by cells to support the rapid proliferation and effector functions. CO2 affects cell metabolism and high levels of CO2 act as toxin in cell cultures. Although the aforementioned factors play a major role in cell culture condition, tissue engineering is currently done in single-use vessels which are not equipped with systems monitoring them. As a result, cell cultures are not usually studied from an analytical point of view, and this makes these types of experiments less repeatable and reliable. The Center for Advanced Sensor Technology has developed noninvasive CO2 and glucose sensors (featuring a sampler mounted outside the vessel), pH and DO sensors (featuring a patch attached to the bottom of the vessel) to monitor cell culture environment. The low profile of the sensors makes them suitable for various kinds of bioreactors. In this study, human cell cultures were conducted in standard cell culture flasks equipped with sensors for DO, pH, DCO2, and glucose for continuous monitoring of process parameters. The analytical data were used to interpret the effect of various microenvironmental conditions on cell behavior. The results show that the use of these sensors improved understanding of the microenvironment and provided real-time information on nutrients and the metabolites for small-scale cell cultures.

Interpreting small-scale human cell culture using noninvasive sensors for dissolved CO2, dissolved O2, pH and glucose

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.