Highly toxic and flammable H2S gas is an environmental threat. Thioalkalivibrio versutus is an autotroph that can transform hydrogen sulfide to sulfur, in the form of extracellular globules via oxidation. Haloalkaliphilic autotrophs, like the bio-desulfurizing T. versutus, grow slowly. Weak growth makes any trial for developing potent genetic tools required for genetic engineering far from achieved. In this study, the fed-batch strategy improved T. versutus growth by 1.6 fold in maximal growth rate, 9-fold in O.D600 values and about 3-fold in biomass and protein productions. The strategy also increased the favorable desulfurization product, sulfur, by 2.7 fold in percent yield and 1.5-fold in diameter. A tight iron-inducible expression system for T. versutus was successfully developed. The system was derived from fed-batch cultivation coupled with new design, build, test and validate (DPTV) approach. The inducible system was validated by toxin expression. Fed-batch culturing coupled with DBTV approach successfully led to the first tight inducible system construction in T. versutus for improving bio-desulfurization processes by future metabolic engineering. The selected inducible system heterologously expressed the MazF toxin at high O.D600 value as a validation step. It lets the strain grow much faster than the control. The cultivation coupled with DPTV approach could be applied to other autotrophs.

Development of growth-driven stepwise inducible expression system in haloalkaliphilic desulfurizing bacteria Thioalkalivibrio versutus

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.