There are many chemical and physical requirements that must be met for antibodies to be suitable for use as therapeutics. One of the most important requirements for therapeutic antibodies is high specificity for their target antigens because off-target binding is correlated with rapid antibody clearance rates and suboptimal pharmacokinetic profiles. Therefore, we have sought to develop high-throughput methods for rapidly evaluating antibody specificity using minimal amounts of purified protein and widely accessible experimental methods. Our flow cytometry approach – which uses multiple types of polyspecificity reagents – is well correlated with other methods for evaluating antibody specificity as well as with assays used for identifying antibodies with abnormal clearance rates <i>in vivo</i>. We expect that these results will improve the rapid evaluation of key antibody biophysical properties linked to their developability and thereby improve the drug development process.

High-throughput evaluation of antibody developability using flow cytometry

Macromolecular Chemistry: The Second Century

[Author Benefits](https://scimeetings.acs.org/?utm_source=pubs_content_marketing&utm_medium=website&utm_campaign=0320_MCF_NPI_Launch_Spring_Homepage&ref=pubs_content_marketing)

[How to use SciMeetings](https://storage.googleapis.com/dl.morressier.com/acs/SciMeetings-ACSSpring2020-HowToGuide_M3020-Final.pdf)

[Frequently Asked Questions](https://storage.googleapis.com/dl.morressier.com/acs/SciMtng_ACSNMSp2020_FAQ_M3120_Final.pdf)

ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.