During the early phases of development, purification of relatively large volumes of media with low levels of recombinant protein is often required. This process is typically inefficient, because pumping large volumes through small chromatography columns is time-consuming. We have evaluated a single pass tangential flow filtration (SPTFF) disposable device that enables on line concentration of feed streams 3 – 9 fold prior to purification. This can have a significant time saving benefit to the first chromatography step and potentially an improvement in overall process productivity. This technique can be applied to all modalities such as IgGs, Fabs, DNA and viral vectors with minimal development effort once membrane compatibility has been established.

Improving the productivity of a purification process using a novel ‘in line’ product concentrator

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.