When developing a biomanufacturing process, understanding the lifetime of a resin is important for estimating the costs associated with the chromatographic purification portion of the process and provides an understanding for how robust the purification process is.  A 300 cycle purification process study which contained 0.1M sodium hydroxide cleaning in place and sanitization with peracetic acid every 100 cycles was investigated using harvested cell culture fluid (HCCF) as load material. Using the same load material, a 50 cycle study was conducted on Chromassette®, a lattice supported modular chromatography platform, packed with the same Protein A chromatography resin.  After the abbreviated life cycle test on Chromassette, the sporicidal impact of PAA on <i>B. subtilis</i> was tested on both a column and Chromassette.  Results show comparability of lifetime and cleanability between Protein A resin packed in a traditional column format and in Chromassette.<br/><br/>

Exploration of cycling and sanitization on lattice supported Protein A chromatography

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.