To implement a Protein A chromatography resin in downstream processing for therapeutic monoclonal antibody, many factors are considered, such as dynamic binding capacity, impurity clearance, and pressure properties. Chemical stability and resin lifetime are important performance attributes for chromatography resin selection to characterize performance consistency and to understand the process cost over the resin’s lifetime. In this study, the chemical stability of a methacrylic resin, Amsphere A3, was evaluated against various cleaning and sanitizing reagents. Furthermore, resin lifetime was also investigated under various process buffer conditions including sodium hydroxide and peracetic acid.

Investigating effective cleaning agents and sanitization protocols for protein A chromatography through chemistry, process characterization and bacterial challenge studies

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.