A continuous affinity precipitation process using elastin-like polypeptides (ELPs) conjugated to the Z domain of protein A was developed to purify mAbs. The setup incorporates binding of the mAb to ELP-Z, precipitation of the complex, capture and washing of the precipitates, and removal of the mAb from the ELP-Z precipitates. This system employs static mixers for efficient mixing during the binding and precipitation steps. Depth filters are used to trap ELPZ-mAb precipitates. The process was optimised for purity, recovery and robustness across multiple mAbs and a control strategy was developed to maintain process efficacy.

Continuous ELP-based affinity precipitation for the purification of therapeutics

Macromolecular Chemistry: The Second Century

[Author Benefits](https://scimeetings.acs.org/?utm_source=pubs_content_marketing&utm_medium=website&utm_campaign=0320_MCF_NPI_Launch_Spring_Homepage&ref=pubs_content_marketing)

[How to use SciMeetings](https://storage.googleapis.com/dl.morressier.com/acs/SciMeetings-ACSSpring2020-HowToGuide_M3020-Final.pdf)

[Frequently Asked Questions](https://storage.googleapis.com/dl.morressier.com/acs/SciMtng_ACSNMSp2020_FAQ_M3120_Final.pdf)

ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.