In the past five years, Pall’s iCELLis bioreactors have emerged as the leading bioreactor technology for clinical manufacturing of viral vectors and vaccines. To date, the majority of products manufactured in the iCELLis bioreactors have utilized serum-containing media formulations that were developed primarily for traditional flatware. These media formulations may not be ideal for three-dimensional culture or for mature processes in clinical manufacturing. InVitria<sup> </sup>has developed OptiPEAK HEK293T, a blood free and chemically defined media optimized for use in the iCELLis bioreactors unique dynamic environment. Chemically defined and blood-free medias provide manufacturers less regulatory barriers to overcome by mitigating the supply chain risk and variability that comes from serum. Adoption of InVitria’s media formulation in the iCELLis bioreactor can result in efficient cell attachment, cell population doubling times comparable to traditional serum-containing medias, and acceptable post seeding growth lags. In this poster, we demonstrate the successful implementation of OptiPEAK HEK293T blood-free media formulation in the iCELLis bioreactor for adherent HEK293T. We begin by demonstrating rapid and uniform cell attachment to the iCELLis fixed-bed bioreactor, followed by robust expansion kinetics found to be equivalent to those observed in serum-containing media. These results enhance the regulatory pathway for product approval by removing supply chain risk and reducing risks associated with highly variable serum composition in large scale clinical manufacturing.

Adherent HEK293T cells cultured in Pall’s iCELLis® nano bioreactor with OptiPEAK HEK293T blood-free chemically defined media exhibit robust and rapid population doubling times

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.