Therapeutic protein N-linked and/or O-linked glycoform characteristics can impact ADCC, CDC, immunogenicity, and other important drug properties. The ability to produce proteins with a wide variety of glycosylation patterns is crucial for the testing and selection of optimal candidates to move from Discovery to Clinical development. This work demonstrates that it is possible to control a variety of glycosylation patterns, through growth medium supplementation, in a CHO transient transfection system. Through chemical supplementation, galactosylation, fucosylation, mannosylation, and arabinosylation were altered, quantified, and compared with an identical protein expressed in a stable CHO cell line and altered through similar medium supplementation. The ability to quickly and controllably express glycoengineered proteins by means of a transient CHO process can aid in speeding candidate selection and time to clinic.<br/>

Rapid production of glycoengineered proteins via CHO transient transfection

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.