<br/>Development of process analytical technology (PAT) tools in parallel with cell culture development provides a mechanism to design, analyze and control biological processes through measurement of process parameters which affect critical quality attributes (CQAs). Some examples of PAT tools used in cell culture processes include capacitance probes and Raman probes, which can provide real-time monitoring of performance parameters such as metabolites, titer, and amino acid concentrations. The goals of this project are to 1) develop methods for online monitoring of metabolites and titer, using in-line Raman probe, and 2) develop in-line capacitance probe enabled real-time monitoring of VCD.<br/>              Capacitance and Raman data is collected from multiple bioreactor runs and used to develop partial least square (PLS) calibration and prediction models for real time monitoring of cell culture process parameters. For example, multiple data points for VCD and metabolites derived from Raman models will enable optimization of the cell culture process to increase productivity and control the variability of the CQAs. In addition, real time monitoring of VCD using capacitance probes will help to streamline the transfer of cells to production bioreactor. In conclusion, PAT tools play a critical role in developing robust cell culture processes. Furthermore, experience gained in developing the PLS models and application at small scale will simplify the process of transferring PAT technology to manufacturing sites.

Use of PAT to facilitate development and tech transfer of a cell culture process

Macromolecular Chemistry: The Second Century

[Author Benefits](https://scimeetings.acs.org/?utm_source=pubs_content_marketing&utm_medium=website&utm_campaign=0320_MCF_NPI_Launch_Spring_Homepage&ref=pubs_content_marketing)

[How to use SciMeetings](https://storage.googleapis.com/dl.morressier.com/acs/SciMeetings-ACSSpring2020-HowToGuide_M3020-Final.pdf)

[Frequently Asked Questions](https://storage.googleapis.com/dl.morressier.com/acs/SciMtng_ACSNMSp2020_FAQ_M3120_Final.pdf)

ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.